Biophysical Journal 1: 215-226 (1961)
© 1961 the Biophysical Society

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Studies of E. coli Ribosomal RNA and Its Degradation Products

ABSTRACT

The RNA of E. coli ribosomes has been extracted by the phenol method. The 70S ribosomes contain RNA molecules of 28 and 18S almost exclusively. When the 70S ribosomes are dissociated to 30 and 50S ribosomes the former contain only the 18S RNA and the latter a mixture of 28 and 18S RNA. There are also present, however, small quantities of ribosomal RNA having sedimentation coefficients of between 4 and 8S. These small molecules are particularly abundant in the smaller ribosomes present in the cell extract and account for most of the RNA of 20S ribosomes.

In addition it has proved possible to degrade the large molecules of RNA to a series of smaller molecules. Removal of magnesium ions from the growing cell, extensive dialysis of the RNA against a buffer of low ionic strength, and heating all resulted in such degradation. Three degradation products were observed having sedimentation coefficients of about 13.1S, 8.8S, and 4.4S. The integral sedimentation distributions of these preparations suggest a high degree of homogeneity among the molecules of each of the three classes. The three sizes seem to result from sequential breaks in the molecules since the proportion of smaller molecules increases with time of treatment.

The molecular weights of the 8.8S and 4.4S molecules have been estimated as 144,000 ± 4,900 and 29,200 ± 1,200 respectively by the Archibald method.