Biophysical Journal 1: 517-523 (1961)
© 1961 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Green, M. H. Articles by Hall, B. D. Search for Related Content PubMed PubMed Citation Articles by Green, M. H. Articles by Hall, B. D.

A Comparison of the Native and Derived 30S and 50S Ribosomes of Escherichia coli

ABSTRACT

Four types of ribosomes occurring in E. coli have been separated by sucrose gradient centrifugation. These are the 30S and 50S particles occurring in E. coli extracts (native particles), and the 30S and 50S particles which are the subunits of 70S ribosomes (derived particles). Two criteria were used in comparing these particles: (1) The type of RNA contained in each, as determined by sedimentation velocity in the analytical ultracentrifuge. (2) The ability of mixtures of 30S and 50S ribosomes (derived 30S + derived 50S, native 30S + native 50S) to undergo the reaction: [Formula: see text] Native and derived 30S particles were found to contain 16S RNA. Derived 50S particles contained 23S RNA and a small amount of 15 to 20S RNA, whereas native 50S ribosomes contained only 16S RNA. Derived 30S and 50S particles combined to form 70S particles. However, under identical conditions, native 30S and 50S particles did not form 70S ribosomes.

This article has been cited by other articles:

				H. R. Henney Jr. and R. Storck Ribosomes and Ribonucleic Acids in Three Morphological States of Neurospora Science, December 27, 1963; 142(3600): 1675 - 1677. [Abstract] [PDF]