Biophysical Journal 1: 639-647 (1961)
© 1961 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boezi, J. A. Articles by Cowie, D. B. Search for Related Content PubMed PubMed Citation Articles by Boezi, J. A. Articles by Cowie, D. B.

Kinetic Studies of ß-Galactosidase Induction

ABSTRACT

The kinetics of ß-galactosidase induction in E. coli ML 3 have been studied. Following addition of inducer, the rate of enzyme synthesis accelerates from the uninduced to a steady-state rate. At saturating concentration of inducer the time constant (T_c) for this process is 2.5 to 3 minutes. With decreasing inducer concentration (I), increasing time constants are observed. I/I + K' approximates I/T_c. The steady-state rate of ß-galactosidase synthesis is approximated by I²/I² + K². K' and K have been estimated for IPTG and TMG. The kinetics of ß-galactosidase production after the removal of inducer by dilution or after the addition of glucose have been investigated. A transition time of 2.5 to 3 minutes is observed before enzyme synthesis slows or stops. These results are consistent with the hypothesis that the enzyme-forming unit is unstable.

This article has been cited by other articles:

				S. Oehler, S. Alberti, and B. Muller-Hill Induction of the lac promoter in the absence of DNA loops and the stoichiometry of induction Nucleic Acids Res., January 23, 2006; 34(2): 606 - 612. [Abstract] [Full Text] [PDF]

				I. Pastan and R. Perlman Cyclic Adenosine Monophosphate in Bacteria: In many bacteria the synthesis of inducible enzymes requires this cyclic nucleotide Science, July 24, 1970; 169(3943): 339 - 344. [Abstract] [PDF]

				E. C. Pollard Ionizing Radiation: Effect on Genetic Transcription Science, November 13, 1964; 146(3646): 927 - 929. [Abstract] [PDF]