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Biophysical Journal 1: 711-721 (1961)
© 1961 the Biophysical Society

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Spectroscopic Evidence for Complexing of Acetic Acid with Bovine Serum Albumin, Gramicidin, and Dimethylformamide

John R. Cann

ABSTRACT

Acetic acid has a major effect on the absorption spectra of bovine serum albumin, gramicidin, and dimethylformamide in the region, 255 to 200 mµ. Increasing the concentration of acetic acid causes progressively decreasing absorbency accompanied by a large and progressively increasing red shift of the absorption maximum. The decrease in absorbency is interpreted in terms of a reversible complexing of acetic acid with these molecules and the red shift in terms of a non-specific solvent effect.







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Copyright © 1961 by the Biophysical Society.