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Biophysical Journal 10: 630-645 (1970)
© 1970 the Biophysical Society

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Density Invariance of Cultured Chinese Hamster Cells with Stage of the Mitotic Cycle

E. C. Anderson, D. F. Petersen and R. A. Tobey

ABSTRACT

Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar measurements on synchronized cultures indicate that reduced density varies by less than 2% around the life cycle. The mean density of CHO cells in F-10 growth medium is calculated to be 1.051 after correction for osmotic effects of the Ficoll gradient.






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Copyright © 1970 by the Biophysical Society.