help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 11: 798-809 (1971)
© 1971 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehnert, S.
Right arrow Articles by Moroson, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehnert, S.
Right arrow Articles by Moroson, H.

Dependence of Single-Stranded DNA Length on Stage of Growth Cycle in Escherichia coli B/r and Effect of Irradiation

Shirley Lehnert and Harold Moroson

ABSTRACT

Alkaline sucrose density gradient profiles of DNA from log phase Escherichia coli B/r (CSH) show a main peak with sedimentation coefficient at approximately 130S and a shoulder or second peak at approximately 90S. Incorporation of radioactive precursors into the 90S peak precedes their appearance in the main peak. The size of the second peak appears to be directly related to the rate of replication and it is not present in profiles of nondividing stationary phase cultures. The decrease in weight average molecular weight (Mw) of DNA produced by X-rays is also directly related to the rate of replication. It is greatest in log phase E. coli B/r and least in stationary phase cells, because of the efficiency of rejoining of radiation-induced single strand breaks in DNA of the latter cells.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the Biophysical Society.