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Biophysical Journal 13: 470-479 (1973)
© 1973 the Biophysical Society

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Temperature Variation of Polyribonucleotide Conformation by an Interaction with Basic Globular Proteins

Dennis A. Stevenson and John W. Preiss

ABSTRACT

Chicken lysozyme interacts with polyribonucleotides to form large light-scattering centers. The size of these is critically dependent on conformation of the polynucleotides. Hence, the interaction provides a very sensitive method of determining temperature intervals within which the polynucleotides change from multistranded, stacked-base structures to single-stranded, random coils. This change can be observed with the unaided eye, especially when concentrations of polynucleotides are of the order of a few micrograms per milliliter.







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Copyright © 1973 by the Biophysical Society.