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Biophysical Journal 15: 233-237 (1975)
© 1975 the Biophysical Society

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Viscoelastic analysis of high molecular weight, alkali-denatured DNA from mouse 3T3 cells.

E L Uhlenhopp

ABSTRACT

Alkaline lysates of mouse 3T3 cells showed viscoelastic properties characteristic of very large molecules of single-stranded DNA. The viscoelastic retardation time and the sensitivity to low doses of nitrogen mustard and of X-irradiation suggest a molecular weight in excess of 10-10 daltons. Contact-inhibited cells yielded larger single strands than actively growing cells.




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D. Hung, L. Marton, D. Deen, and R. Shafer
Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells
Science, July 22, 1983; 221(4608): 368 - 370.
[Abstract] [PDF]


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Copyright © 1975 by the Biophysical Society.