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Biophysical Journal 15: 455-467 (1975)
© 1975 the Biophysical Society

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Polarization from a Helix of Fluorophores and its Relation to that Obtained from Muscle

Richard T. Tregear and Robert A. Mendelson

ABSTRACT

Expressions for the polarization of fluorescence from fluorophores in a helical array have been obtained. These predictions have been compared with experimental data obtained by reacting fluorescent S-1 subfragments of myosin (extrinsically labeled with N-(iodoacetylaminoethyl)-5-napthylamine-1-sulfonic acid [1,5-IAEDANS]) with glycerated rabbit psoas fibers in rigor. A comparison with data obtained by directly labeling fibers with this fluorophore is also presented.




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Yu. S. Borovikov, I. V. Dedova, C. G. dos Remedios, N. N. Vikhoreva, P. G. Vikhorev, S. V. Avrova, T. L. Hazlett, and B. W. Van Der Meer
Fluorescence Depolarization of Actin Filaments in Reconstructed Myofibers: The Effect of S1 or pPDM-S1 on Movements of Distinct Areas of Actin
Biophys. J., May 1, 2004; 86(5): 3020 - 3029.
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Copyright © 1975 by the Biophysical Society.