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Biophysical Journal 15: 753-763 (1975)
© 1975 the Biophysical Society

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Iontophoresis of cyclic AMP.

M H Cohen, D J Drage and A Robertson

ABSTRACT

The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.




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Copyright © 1975 by the Biophysical Society.