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Biophysical Journal 16: 883-889 (1976)
© 1976 the Biophysical Society

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Rate of DNA chain elongation in ultraviolet light-irradiated mammalian cells as estimated by a bromodeoxyuridine photolysis method.

L F Povirk and R B Painter

ABSTRACT

Using a new method, we show that, when the DNA chain elongation rate is measured for short time periods, ultraviolet light does not decrease this rate. The method is based on the photolysis of bromouracil-containing DNA by 313 nm light and alkaline sucrose gradients.






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Copyright © 1976 by the Biophysical Society.