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Biophysical Journal 2: 1-9 (1962)
© 1962 the Biophysical Society

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The Molecular Weight and Other Biophysical Properties of Bromegrass Mosaic Virus

Larry E. Bockstahler and Paul Kaesberg

ABSTRACT

Data are presented which show that bromegrass mosaic virus has a particularly low molecular weight and nucleic acid content. A molecular weight of 4.6 x 106 was calculated from the sedimentation coefficient, S°20,w = 86.2S, the diffusion coefficient, D20,w = 1.55 x 10-7 cm2/sec., and an assumed partial specific volume, [UNK] = 0.708 ml/gm. The virus has a ribonucleic acid content of 1.0 x 106 atomic mass units. Electrophoresis experiments showed that the virus is stable in 0.10 ionic strength buffers in the pH range 3-6. Breakdown of the virus was observed outside this pH range. Some characteristics of the breakdown products are described.






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Copyright © 1962 by the Biophysical Society.