Biophysical Journal 2: 129-141 (1962)
© 1962 the Biophysical Society

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Effect of Concentration on the Formation of Molecular Hybrids from T₄ DNA

ABSTRACT

When the thymine of T₄ DNA is replaced by 5-BU the melting temperature of T₄ DNA is increased from about 83° to about 93°C. Heating and slow cooling of T₄ DNA at concentrations of about 30 µg/ml leads to aggregates which consist of several polynucleotide chains which appear in the electron microscope as a branched structure. The aggregates have regions which are true hybrids. When the concentration of T₄ DNA is lowered to less than 1 µg/ml the products of hybridization are not aggregates but have the morphology of native DNA molecules and the density labels are distributed as expected from the fusing of two chains of approximately equal length.