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Biophysical Journal 2: 433-449 (1962)
© 1962 the Biophysical Society

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The Adsorption of Bacteriophage {phi}X174 to its Host

Robert Fujimura and Paul Kaesberg

ABSTRACT

The adsorption of purified {phi}X174 to E. coli C and to E. coli C cell walls was investigated. Adsorption was measured by assaying for unadsorbed plaque formers. The amount of irreversible and reversible adsorption depends upon pH and divalent ion concentration. Maximum irreversible adsorption occurs in 0.1 M CaCl2 at 36°C. There is no detectable reversible adsorption at conditions of pH and CaCl2 concentration optimum for irreversible adsorption. Under these optimum conditions, diffusion is not the rate-limiting factor, and the encounter efficiency appears to be low. The rate constant is 1.0 x 10-10 ml/sec. Phages adsorbed irreversibly to live cells cause infection and to the isolated cell walls apparently cause release of DNA. There is a specific {phi}X174 receptor site on the mucocomplex portion of the cell wall.






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Copyright © 1962 by the Biophysical Society.