Biophysical Journal 2: 451-463 (1962)
© 1962 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Person, S. Articles by Leah Lewis, H. Search for Related Content PubMed Articles by Person, S. Articles by Leah Lewis, H.

Effects of Decay of Incorporated H³-Thymidine on Bacteria

ABSTRACT

The killing efficiency due to the decay of incorporated H³-thymidine in three mutants of E. coli strain 15: 15_T-, 15_T-L-, and 15_T-U- has been determined. This efficiency is comparable to that previously determined by others for P³² decay. The killing efficiency has been determined as a function of H³-thymidine specific activity, storage media and storage temperature. We have observed a latent killing effect that causes lethality under certain conditions. The kinetics of latent killing have been examined at several temperatures. Finally, mutation production induced by H³-thymidine decays was shown to occur. The results are consistent with the idea that inactivation and mutations may be caused by a process in the nuclear transmutation that is not associated with ß-particle ionization damage.

This article has been cited by other articles:

				S. Person and M. Osborn DNA Segregation in Escherichia coli: Observations by Means of Tritiated Thymidine Decay Science, January 3, 1964; 143(3601): 44 - 46. [Abstract] [PDF]