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Biophysical Journal 23: 7-13 (1978)
© 1978 the Biophysical Society

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Hydrodynamic orientation of sperm heads for flow cytometry.

P N Dean, D Pinkel and M L Mendelsohn

ABSTRACT

Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.




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Home page
 Hum ReprodHome page
U.B. Hacker-Klom, W. Gohde, E. Nieschlag, and H.M. Behre
DNA flow cytometry of human semen
Hum. Reprod., October 1, 1999; 14(10): 2506 - 2512.
[Abstract] [Full Text] [PDF]


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Copyright © 1978 by the Biophysical Society.