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Biophysical Journal 23: 463-471 (1978)
© 1978 the Biophysical Society

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Changes of intracellular Ca++ as measured by arsenazo III in relation to the K permeability of human erythrocyte ghosts.

D R Yingst and J F Hoffman

ABSTRACT

A Ca-sensitive dye, arsenazo III, has been incorporated into resealed human erythrocyte ghosts and calibrated to monitor continuously micromolar concentrations of intracellular ionized Ca ([Ca++]i). When the external concentration of Ca is much greater than [Ca++]i, [Ca++]i increases because of a net balance between Ca influx and efflux. Dynamic changes in [Ca++]i regulate K efflux, which in turn may influence the rate of Ca influx. A procedure for purifying arsenazo III is also described.






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Copyright © 1978 by the Biophysical Society.