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Biophysical Journal 25: 465-472 (1979)
© 1979 the Biophysical Society

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Bacteriorhodopsin in model membranes. A new component of the displacement photocurrent in the microsecond time scale.

F T Hong and M Montal

ABSTRACT

A quasi-short-circuit (tunable voltage clamp) measurement method with microsecond time resolution was applied to a bacteriorhodopsin model membrane formed by a novel interfacial technique. A new component (B1) of the displacement photocurrent was recorded: it has no detectable latency at an instrumental time constant of 1.5 museconds, and persists at 5 degrees C. In addition, a slower component (B2) of opposite polarity inhibited by low temperature (5 degrees C) and low pH (pH = 3.0) was recorded. The technique is very sensitive for the study of fast capacitative photoresponses in model membranes, and allows the detection of charge displacements in bacteriorhodopsin associated with distinct stages of the photochemical transformation.






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Copyright © 1979 by the Biophysical Society.