| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 26: 115-131 (1979)
© 1979 the Biophysical Society
ABSTRACT
The elastic properties of the human red blood cell membrane have been measured as functions of temperature. The area compressibility modulus and the elastic shear modulus, which together characterize the surface elastic behavior of the membrane, have been measured over the temperature range of 2-50 degrees C with micropipette aspiration of flaccid and osmotically swollen red cells. In addition, the fractional increase in membrane surface area from 2-50 degrees C has been measured to give a value for the thermal area expansivity. The value of the elastic shear modulus at 25 degrees C was measured to be 6.6 X 10(-3) dyne/cm. The change in the elastic shear modulus with temperature was -6 X 10(-5) dyne/cm degrees C. Fractional forces were shown to be only on the order of 10-15%. The area compressibility modulus at 25 degrees C was measured to be 450 dyne/cm. The change in the area compressibility modulus with temperature was -6 dyne/cm degrees C. The thermal area expansivity for red cell membrane was measured to be 1.2 X 10(-3)/degrees C. With this data and thermoelastic relations the heat of expansion is determined to be 110-200 ergs/cm2; the heat of extension is 2 X 10(-2) ergs/cm2 for unit extension of the red cell membrane. The heat of expansion is of the order anticipated for a lipid bilayer idealized as twice the behavior of a monolayer at an oil-water interface. The observation that the heat of extension is positive demonstrates that the entropy of the material increases with extension, and that the dominant mechanism of elastic energy storage is energetic. Assuming that the red cell membrane shear rigidity is associated with "spectrin," unit extension of the membrane increases the configurational entropy of spectrin by 500 cal/mol.
This article has been cited by other articles:
 |
|
 |
|
  Y. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum PNAS, September 16, 2008; 105(37): 13730 - 13735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Butler, N. Mohandas, and R. E. Waugh Integral Protein Linkage and the Bilayer-Skeletal Separation Energy in Red Blood Cells Biophys. J., August 15, 2008; 95(4): 1826 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Mirijanian and G. A. Voth Unique elastic properties of the spectrin tetramer as revealed by multiscale coarse-grained modeling PNAS, January 29, 2008; 105(4): 1204 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Liu, C. J. Goergen, and J.-Y. Shao Effect of Temperature on Tether Extraction, Surface Protrusion, and Cortical Tension of Human Neutrophils Biophys. J., October 15, 2007; 93(8): 2923 - 2933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Solov'yov and W. Greiner Theoretical Analysis of an Iron Mineral-Based Magnetoreceptor Model in Birds Biophys. J., September 1, 2007; 93(5): 1493 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paramore, G. S. Ayton, D. T. Mirijanian, and G. A. Voth Extending a Spectrin Repeat Unit. I: Linear Force-Extension Response Biophys. J., January 1, 2006; 90(1): 92 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Marcelli, K. H. Parker, and C. P. Winlove Thermal Fluctuations of Red Blood Cell Membrane via a Constant-Area Particle-Dynamics Model Biophys. J., October 1, 2005; 89(4): 2473 - 2480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cuvelier, I. Derenyi, P. Bassereau, and P. Nassoy Coalescence of Membrane Tethers: Experiments, Theory, and Applications Biophys. J., April 1, 2005; 88(4): 2714 - 2726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pierrat, F. Brochard-Wyart, and P. Nassoy Enforced Detachment of Red Blood Cells Adhering to Surfaces: Statics and Dynamics Biophys. J., October 1, 2004; 87(4): 2855 - 2869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Law, G. Liao, S. Harper, G. Yang, D. W. Speicher, and D. E. Discher Pathway Shifts and Thermal Softening in Temperature-Coupled Forced Unfolding of Spectrin Domains Biophys. J., November 1, 2003; 85(5): 3286 - 3293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hategan, R. Law, S. Kahn, and D. E. Discher Adhesively-Tensed Cell Membranes: Lysis Kinetics and Atomic Force Microscopy Probing Biophys. J., October 1, 2003; 85(4): 2746 - 2759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Law, P. Carl, S. Harper, P. Dalhaimer, D. W. Speicher, and D. E. Discher Cooperativity in Forced Unfolding of Tandem Spectrin Repeats Biophys. J., January 1, 2003; 84(1): 533 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Hochmuth and W. D. Marcus Membrane Tethers Formed from Blood Cells with Available Area and Determination of Their Adhesion Energy Biophys. J., June 1, 2002; 82(6): 2964 - 2969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ra, C Picart, H Feng, H. Sweeney, and D. Discher Muscle cell peeling from micropatterned collagen: direct probing of focal and molecular properties of matrix adhesion J. Cell Sci., January 5, 1999; 112(10): 1425 - 1436. [Abstract] [PDF]
|
|
|
|
 |
|
 A Elgsaeter, B. Stokke, A Mikkelsen, and D Branton The molecular basis of erythrocyte shape Science, December 5, 1986; 234(4781): 1217 - 1223. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
