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Biophysical Journal 29: 367-378 (1980)
© 1980 the Biophysical Society

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Analysis of nonexponential fluorescence decay data by a method of moments.

T N Solie, E W Small and I Isenberg

ABSTRACT

A method of moments is presented for the analysis of convoluted fluorescence decay data when the impulse response function is given by f(t) = alpha exp (-At - Bt1/2). Examples of this method are given using both simulated and measured fluorescence decays. It is also shown that this method, used with moment index displacements, will correct for light-scatter leakage, zero-point time shifts, and slow lamp drift.






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Copyright © 1980 by the Biophysical Society.