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Biophysical Journal 3: 1-10 (1963)
© 1963 the Biophysical Society

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Characterization of Ribosomes from Neurospora crassa

R. Storck

ABSTRACT

Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20°C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl2 but undergo a dissociation into smaller particles if the MgCl2 concentration is lowered to 0.0001 M. Two types of RNA with sedimentation coefficients of 19S2050 and 13S2050 have been extracted from the 81S particles.




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H. R. Henney Jr. and R. Storck
Ribosomes and Ribonucleic Acids in Three Morphological States of Neurospora
Science, December 27, 1963; 142(3600): 1675 - 1677.
[Abstract] [PDF]




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Copyright © 1963 by the Biophysical Society.