Biophysical Journal 3: 49-63 (1963)
© 1963 the Biophysical Society

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Physicochemical Studies on the Reaction between Formaldehyde and DNA

ABSTRACT

The reaction between formaldehyde and phage T7 DNA has been studied by optical absorbance and sedimentation measurements. Through the course of denaturation, OD₂₀₀ and s_{20, w} rise; after the attainment of full hyperchromicity the s_{20, w} falls sharply, suggesting a decrease in molecular weight. Conditions in which formaldehyde causes cross-linking are defined. Some experimental applications of the denaturation technique are given. Evidence which suggests that preformed single-strand interruptions may exist in phage DNA is briefly discussed.

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