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Biophysical Journal 3: 115-125 (1963)
© 1963 the Biophysical Society

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Low-Angle Light Scattering of Deoxyribonucleic Acid

D. Froelich, C. Strazielle, G. Bernardi and H. Benoît

ABSTRACT

An instrument of new design has been built in order to perform lowangle light-scattering measurements to angles as low as 16°. Native deoxyribonucleic acid preparations of different molecular weights have been studied using this apparatus with a new clarification technique. The molecular weights obtained from the low-angle data have been compared with those calculated for the same samples when using the results in the 30-150° range. The two sets of data yield the same molecular weights up to values of about 6 x 106. Higher molecular weights are underestimated to a variable extent when measurements in the usual angular region (30-150°) are used.






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Copyright © 1963 by the Biophysical Society.