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Biophysical Journal 3: 189-197 (1963)
© 1963 the Biophysical Society

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Study of the Radiosensitive Structure of T2 Bacteriophage Using Low Energy Electron Beams

A. Cole and R. Langley

ABSTRACT

Hydrated T2 bacteriophage were irradiated with 0.75 to 90 kev electron beams. A thin foil isolated the sample chamber from the electron gun source. Survival (plaque formation) was observed. Apparent cross-sections and D37 doses were determined. The maximum cross-section of about 5 x 10-3 µ2 is roughly equal to the cross-sectional area presented by the phage core. As beam energy was increased the average D37 dose first attained a minimum value of about 23 kr for 1 kev electrons (which penetrate the relatively inert protein coat) after which the average D37 dose rose with beam energy to a maximum value of about 50 kr for fully penetrating beams. These dependencies suggest that the radiosensitive structure exists as a peripheral shell rather than a uniformly sensitive core. A tentative model for the phage structure, based on this and other evidence, is presented.






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Copyright © 1963 by the Biophysical Society.