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Biophysical Journal 30: 181-186 (1980)
© 1980 the Biophysical Society

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Intracellular Na+ activity as a function of Na+ transport rate across a tight epithelium.

N K Wills and S A Lewis

ABSTRACT

Liquid Na+ resin microelectrodes were used to measure intracellular Na+ activities (alpha iNa+) in the mammalian tight epithelium, rabbit urinary bladder. alpha iNa+ averaged 7 +/- 1 mM and was independent of Na+ transport rate over the range of 2 to 8 muA/muF. (1 mF congruent to 1 cm2 apical membrane area). After Na+ loading the cells the Na+ pump in the basolateral membrane was measurably electrogenic. A method for shielding the Na+-sensitive microelectrodes is described which increases the response time and eliminates an electrical shunting artifact.






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Copyright © 1980 by the Biophysical Society.