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Biophysical Journal 30: 351-358 (1980)
© 1980 the Biophysical Society

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A laser Raman spectroscopic study of Ca2+ binding to troponin C.

E B Carew, P C Leavis, H E Stanley and J Gergely

ABSTRACT

Laser Raman spectroscopy has been used detect structural changes in troponin C induced by Ca2+ binding. Addition of Ca2+ - Mg2+ sites produces perturbations in the amide III region of the spectrum indicative of increased alpha-helical content, and in regions of the spectrum corresponding to carboxylate, thiol, and phenol side chains. However, Ca2+ binding to the low affinity Ca2+ - specific sites is not detected by laser Raman spectral changes.






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Copyright © 1980 by the Biophysical Society.