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Biophysical Journal 35: 237-242 (1981)
© 1981 the Biophysical Society

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Sarcomere distribution patterns in single cardiac cells.

N M De Clerck, V A Claes, E R Van Ocken and D L Brutsaert

ABSTRACT

Sarcomeres of single cardiac cells isolated either by microdissection or by enzymatic dissociation were visualized on a television screen, through the objective (63 X) of an inverted microscope and a television camera. A distinct line of the television picture was positioned on the preparation and the frequency content, corresponding to the dark and light areas of the striations was tracked by a phase-locked loop. This technique permitted the measurement of the length of successive sarcomeres and hence the sarcomere distribution pattern over the entire preparation.






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Copyright © 1981 by the Biophysical Society.