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Biophysical Journal 35: 455-462 (1981)
© 1981 the Biophysical Society

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Chloride permeability of membrane vesicles isolated from Torpedo californica electroplax.

M M White and C Miller

ABSTRACT

The Cl- permeability of membrane vesicles prepared from the electric organ of the marine ray Torpedo californica was studied by means of radioactive tracer exchange and by measuring the changes in the scattered-light intensity caused by osmotically induced volume changes. Both types of experiments indicate that a substantial fraction of the vesicles is extremely permeable of Cl-. Furthermore, this permeability pathway is inhibited by 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene, a well-known inhibitory of anion transport in a variety of systems. The properties of this permeability pathway are consistent with its identification as the voltage-aged Cl- channel studied in planar bilayers.




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T.-Y. Chen and T.-C. Hwang
CLC-0 and CFTR: Chloride Channels Evolved From Transporters
Physiol Rev, April 1, 2008; 88(2): 351 - 387.
[Abstract] [Full Text] [PDF]




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Copyright © 1981 by the Biophysical Society.