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Biophysical Journal 35: 783-798 (1981)
© 1981 the Biophysical Society

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Testing of aggregation measurement techniques for intramembranous particles.

J T Donnell, 2nd and L X Finegold

ABSTRACT

Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggregations. A simple and adequate technique for quantifying the degree of aggregation, which is practical for the electron microscopist, is presented.






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Copyright © 1981 by the Biophysical Society.