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Biophysical Journal 36: 575-588 (1981)
© 1981 the Biophysical Society

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Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.

M L Johnson, J J Correia, D A Yphantis and H R Halvorson

ABSTRACT

Least-squares analysis of experimental data from the analytical ultracentrifuge is discussed in detail, with particular attention to the use of interference optics in studying nonideal self-associating macromolecular systems. Several samples are given that describe the application of the technique, the expected precision of the results, and some of its limitations. A FORTRAN IV computer program is available from the authors.




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