| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 36: 575-588 (1981)
© 1981 the Biophysical Society
ABSTRACT
Least-squares analysis of experimental data from the analytical ultracentrifuge is discussed in detail, with particular attention to the use of interference optics in studying nonideal self-associating macromolecular systems. Several samples are given that describe the application of the technique, the expected precision of the results, and some of its limitations. A FORTRAN IV computer program is available from the authors.
This article has been cited by other articles:
![]() |
D. Eggink, C. E. Baldwin, Y. Deng, J. P. M. Langedijk, M. Lu, R. W. Sanders, and B. Berkhout Selection of T1249-Resistant Human Immunodeficiency Virus Type 1 Variants J. Virol., July 1, 2008; 82(13): 6678 - 6688. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Gaetani, S. Mootien, S. Harper, P. G. Gallagher, and D. W. Speicher Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site Blood, June 15, 2008; 111(12): 5712 - 5720. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. K. Hayashi, H. M. Ames, and Y. Hayashi Tetrameric Hub Structure of Postsynaptic Scaffolding Protein Homer. J. Neurosci., August 15, 2006; 26(33): 8492 - 8501. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. V. Patel, K. A. Vyas, R. Savtchenko, and S. Roseman The Monomer/Dimer Transition of Enzyme I of the Escherichia coli Phosphotransferase System J. Biol. Chem., June 30, 2006; 281(26): 17570 - 17578. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. V. Patel, K. A. Vyas, R. L. Mattoo, M. Southworth, F. B. Perler, D. Comb, and S. Roseman Properties of the C-terminal Domain of Enzyme I of the Escherichia coli Phosphotransferase System J. Biol. Chem., June 30, 2006; 281(26): 17579 - 17587. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. H. Lawrence, K. B. Luther, H. Schindelin, and J. G. Ferry Structural and Functional Studies Suggest a Catalytic Mechanism for the Phosphotransacetylase from Methanosarcina thermophila J. Bacteriol., February 1, 2006; 188(3): 1143 - 1154. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. D. Auerbach and K. A. Johnson Alternating Site ATPase Pathway of Rat Conventional Kinesin J. Biol. Chem., November 4, 2005; 280(44): 37048 - 37060. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Li, K. Zhao, B. Ossareh-Nazari, G. Da, C. Dargemont, and R. Marmorstein Structural Basis for Interaction between the Ubp3 Deubiquitinating Enzyme and Its Bre5 Cofactor J. Biol. Chem., August 12, 2005; 280(32): 29176 - 29185. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. R. Suntoke and D. C. Chan The Fusion Activity of HIV-1 gp41 Depends on Interhelical Interactions J. Biol. Chem., May 20, 2005; 280(20): 19852 - 19857. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. S. Song, A. Daily, M. G. Fried, M. A. Juliano, L. Juliano, and L. B. Hersh Mutation of Active Site Residues of Insulin-degrading Enzyme Alters Allosteric Interactions J. Biol. Chem., May 6, 2005; 280(18): 17701 - 17706. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. C. Borges, H. Fischer, A. F. Craievich, and C. H. I. Ramos Low Resolution Structural Study of Two Human HSP40 Chaperones in Solution: DJA1 FROM SUBFAMILY A AND DJB4 FROM SUBFAMILY B HAVE DIFFERENT QUATERNARY STRUCTURES J. Biol. Chem., April 8, 2005; 280(14): 13671 - 13681. [Abstract] [Full Text] [PDF] |
||||
![]() |
I. G. Duggin, J. M. Matthews, N. E. Dixon, R. G. Wake, and J. P. Mackay A Complex Mechanism Determines Polarity of DNA Replication Fork Arrest by the Replication Terminator Complex of Bacillus subtilis J. Biol. Chem., April 1, 2005; 280(13): 13105 - 13113. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. B. Gilcrease, D. A. Winn-Stapley, F. C. Hewitt, L. Joss, and S. R. Casjens Nucleotide Sequence of the Head Assembly Gene Cluster of Bacteriophage L and Decoration Protein Characterization J. Bacteriol., March 15, 2005; 187(6): 2050 - 2057. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Chattopadhyay, N. G. Jones, D. Nietlispach, P. R. Nielsen, H. P. Voorheis, H. R. Mott, and M. Carrington Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2 J. Biol. Chem., February 25, 2005; 280(8): 7228 - 7235. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Andrews, J. S. Butler, J. Al-Bassam, L. Joss, D. A. Winn-Stapley, S. Casjens, and G. Cingolani Bacteriophage P22 Tail Accessory Factor GP26 Is a Long Triple-stranded Coiled-coil J. Biol. Chem., February 18, 2005; 280(7): 5929 - 5933. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. S. Song, M. A. Juliano, L. Juliano, M. G. Fried, S. L. Wagner, and L. B. Hersh ATP Effects on Insulin-degrading Enzyme Are Mediated Primarily through Its Triphosphate Moiety J. Biol. Chem., December 24, 2004; 279(52): 54216 - 54220. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. T. Mackey, L. R. Sproul, C. A. Sontag, L. L. Satterwhite, J. J. Correia, and S. P. Gilbert Mechanistic Analysis of the Saccharomyces cerevisiae Kinesin Kar3 J. Biol. Chem., December 3, 2004; 279(49): 51354 - 51361. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. E. Baldwin, R. W. Sanders, Y. Deng, S. Jurriaans, J. M. Lange, M. Lu, and B. Berkhout Emergence of a Drug-Dependent Human Immunodeficiency Virus Type 1 Variant during Therapy with the T20 Fusion Inhibitor J. Virol., November 15, 2004; 78(22): 12428 - 12437. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. J. Y. Simpson, S. H. Yi Lee, N. Bartle, E. Y. Sum, J. E. Visvader, J. M. Matthews, J. P. Mackay, and M. Crossley A Classic Zinc Finger from Friend of GATA Mediates an Interaction with the Coiled-coil of Transforming Acidic Coiled-coil 3 J. Biol. Chem., September 17, 2004; 279(38): 39789 - 39797. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. S. Solovyova, M. Nollmann, T. J. Mitchell, and O. Byron The Solution Structure and Oligomerization Behavior of Two Bacterial Toxins: Pneumolysin and Perfringolysin O Biophys. J., July 1, 2004; 87(1): 540 - 552. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. J. Dubin, P. H. Stokes, E. Y. M. Sum, R. S. Williams, V. A. Valova, P. J. Robinson, G. J. Lindeman, J. N. M. Glover, J. E. Visvader, and J. M. Matthews Dimerization of CtIP, a BRCA1- and CtBP-interacting Protein, Is Mediated by an N-terminal Coiled-coil Motif J. Biol. Chem., June 25, 2004; 279(26): 26932 - 26938. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Tripet, M. W. Howard, M. Jobling, R. K. Holmes, K. V. Holmes, and R. S. Hodges Structural Characterization of the SARS-Coronavirus Spike S Fusion Protein Core J. Biol. Chem., May 14, 2004; 279(20): 20836 - 20849. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. C. Kwok and R. S. Hodges Stabilizing and Destabilizing Clusters in the Hydrophobic Core of Long Two-stranded {alpha}-Helical Coiled-coils J. Biol. Chem., May 14, 2004; 279(20): 21576 - 21588. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Sunde, K. C. Y. McGrath, L. Young, J. M. Matthews, E. L. Chua, J. P. Mackay, and A. K. Death TC-1 Is a Novel Tumorigenic and Natively Disordered Protein Associated with Thyroid Cancer Cancer Res., April 15, 2004; 64(8): 2766 - 2773. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Barkley, R. M. Cornish, and C. D. Poulter Identification of an Archaeal Type II Isopentenyl Diphosphate Isomerase in Methanothermobacter thermautotrophicus J. Bacteriol., March 15, 2004; 186(6): 1811 - 1817. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. B. Tilton, J. M. Shockey, and J. Browse Biochemical and Molecular Characterization of ACH2, an Acyl-CoA Thioesterase from Arabidopsis thaliana J. Biol. Chem., February 27, 2004; 279(9): 7487 - 7494. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Wang, R. Coulombe, D. R. Cameron, L. Thauvette, M.-J. Massariol, L. M. Amon, D. Fink, S. Titolo, E. Welchner, C. Yoakim, et al. Crystal Structure of the E2 Transactivation Domain of Human Papillomavirus Type 11 Bound to a Protein Interaction Inhibitor J. Biol. Chem., February 20, 2004; 279(8): 6976 - 6985. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Grimshaw, H. R. Mott, K. M. Stott, P. R. Nielsen, K. A. Evetts, L. J. Hopkins, D. Nietlispach, and D. Owen Structure of the Sterile {alpha} Motif (SAM) Domain of the Saccharomyces cerevisiae Mitogen-activated Protein Kinase Pathway-modulating Protein STE50 and Analysis of Its Interaction with the STE11 SAM J. Biol. Chem., January 16, 2004; 279(3): 2192 - 2201. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. Cao, C. Bracken, N. R. Kallenbach, and M. Lu Helix formation and the unfolded state of a 52-residue helical protein Protein Sci., January 1, 2004; 13(1): 177 - 189. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Min, H. Kasahara, D. L. Bedgar, B. Youn, P. K. Lawrence, D. R. Gang, S. C. Halls, H. Park, J. L. Hilsenbeck, L. B. Davin, et al. Crystal Structures of Pinoresinol-Lariciresinol and Phenylcoumaran Benzylic Ether Reductases and Their Relationship to Isoflavone Reductases J. Biol. Chem., December 12, 2003; 278(50): 50714 - 50723. [Abstract] [Full Text] [PDF] |
||||
![]() |
T.-S. Tsao, E. Tomas, H. E. Murrey, C. Hug, D. H. Lee, N. B. Ruderman, J. E. Heuser, and H. F. Lodish Role of Disulfide Bonds in Acrp30/Adiponectin Structure and Signaling Specificity: DIFFERENT OLIGOMERS ACTIVATE DIFFERENT SIGNAL TRANSDUCTION PATHWAYS J. Biol. Chem., December 12, 2003; 278(50): 50810 - 50817. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Huang, R. L. Rich, D. G. Myszka, and H. Wu Requirement of Both the Second and Third BIR Domains for the Relief of X-linked Inhibitor of Apoptosis Protein (XIAP)-mediated Caspase Inhibition by Smac J. Biol. Chem., December 5, 2003; 278(49): 49517 - 49522. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Angelaccio, R. Chiaraluce, V. Consalvi, B. Buchenau, L. Giangiacomo, F. Bossa, and R. Contestabile Catalytic and Thermodynamic Properties of Tetrahydromethanopterin-dependent Serine Hydroxymethyltransferase from Methanococcus jannaschii J. Biol. Chem., October 24, 2003; 278(43): 41789 - 41797. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. J. Westman, J. Perdomo, M. Sunde, M. Crossley, and J. P. Mackay The C-terminal Domain of Eos Forms a High Order Complex in Solution J. Biol. Chem., October 24, 2003; 278(43): 42419 - 42426. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. C. Kwok and R. S. Hodges Clustering of Large Hydrophobes in the Hydrophobic Core of Two-stranded {alpha}-Helical Coiled-Coils Controls Protein Folding and Stability J. Biol. Chem., September 12, 2003; 278(37): 35248 - 35254. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. C. Borges, H. Fischer, A. F. Craievich, L. D. Hansen, and C. H. I. Ramos Free Human Mitochondrial GrpE Is a Symmetric Dimer in Solution J. Biol. Chem., September 12, 2003; 278(37): 35337 - 35344. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. B. Christensen, J. M. Colacino, and C. Bonaventura Functional and Biochemical Properties of the Hemoglobins of the Burrowing Brittle Star Hemipholis elongata Say (Echinodermata, Ophiuroidea) Biol. Bull., August 1, 2003; 205(1): 54 - 65. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. D. Fisher, B. Wang, S. L. Alam, D. S. Higginson, H. Robinson, W. I. Sundquist, and C. P. Hill Structure and Ubiquitin Binding of the Ubiquitin-interacting Motif J. Biol. Chem., August 1, 2003; 278(31): 28976 - 28984. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. J. Y. Simpson, E. D. Cram, R. Czolij, J. M. Matthews, M. Crossley, and J. P. Mackay CCHX Zinc Finger Derivatives Retain the Ability to Bind Zn(II) and Mediate Protein-DNA Interactions J. Biol. Chem., July 18, 2003; 278(30): 28011 - 28018. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Hawrani, C. E. Dempsey, M. J. Banfield, D. J. Scott, A. R. Clarke, and B. Kenny Effect of Protein Kinase A-mediated Phosphorylation on the Structure and Association Properties of the Enteropathogenic Escherichia coli Tir Virulence Protein J. Biol. Chem., July 3, 2003; 278(28): 25839 - 25846. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Stray and J. E. Lindsley Biochemical Analysis of the Yeast Condensin Smc2/4 Complex: AN ATPase THAT PROMOTES KNOTTING OF CIRCULAR DNA J. Biol. Chem., July 3, 2003; 278(28): 26238 - 26248. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. A. Plambeck, A. H. Y. Kwan, D. J. Adams, B. J. Westman, L. van der Weyden, R. L. Medcalf, B. J. Morris, and J. P. Mackay The Structure of the Zinc Finger Domain from Human Splicing Factor ZNF265 Fold J. Biol. Chem., June 13, 2003; 278(25): 22805 - 22811. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. S. Kiss, P. M. M. Weers, V. Narayanaswami, J. Cohen, C. M. Kay, and R. O. Ryan Structure-guided Protein Engineering Modulates Helix Bundle Exchangeable Apolipoprotein Properties J. Biol. Chem., June 6, 2003; 278(24): 21952 - 21959. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. F. Mehl, L. D. Heskett, S. S. Jain, and B. Demeler Insights into dimerization and four-helix bundle formation found by dissection of the dimer interface of the GrpE protein from Escherichia coli Protein Sci., June 1, 2003; 12(6): 1205 - 1215. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. M. S. Baker and L. B. Poole Catalytic Mechanism of Thiol Peroxidase from Escherichia coli. SULFENIC ACID FORMATION AND OVEROXIDATION OF ESSENTIAL CYS61 J. Biol. Chem., March 7, 2003; 278(11): 9203 - 9211. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. J. Rasimas, A. E. Pegg, and M. G. Fried DNA-binding Mechanism of O6-Alkylguanine-DNA Alkyltransferase. EFFECTS OF PROTEIN AND DNA ALKYLATION ON COMPLEX STABILITY J. Biol. Chem., February 28, 2003; 278(10): 7973 - 7980. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. R. Hantgan, D. S. Lyles, T. C. Mallett, M. Rocco, C. Nagaswami, and J. W. Weisel Ligand Binding Promotes the Entropy-driven Oligomerization of Integrin alpha IIbbeta 3 J. Biol. Chem., January 24, 2003; 278(5): 3417 - 3426. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. D. Brokx, E. Bolewska-Pedyczak, and J. Gariepy A Stable Human p53 Heterotetramer Based on Constructive Charge Interactions within the Tetramerization Domain J. Biol. Chem., January 17, 2003; 278(4): 2327 - 2332. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Sun, M. Nukaga, K. Mayama, E. H. Braswell, and J. R. Knox Comparison of {beta}-lactamases of classes A and D: 1.5-A crystallographic structure of the class D OXA-1 oxacillinase Protein Sci., January 1, 2003; 12(1): 82 - 91. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Gell, Y. Kong, S. A. Eaton, M. J. Weiss, and J. P. Mackay Biophysical Characterization of the alpha -Globin Binding Protein alpha -Hemoglobin Stabilizing Protein J. Biol. Chem., October 18, 2002; 277(43): 40602 - 40609. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. A. Paulucci, L. Hicks, A. Machado, M. T. M. Miranda, C. M. Kay, and C. S. Farah Specific Sequences Determine the Stability and Cooperativity of Folding of the C-terminal Half of Tropomyosin J. Biol. Chem., October 11, 2002; 277(42): 39574 - 39584. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. S. Carr, G. N. George, and D. R. Winge Yeast Cox11, a Protein Essential for Cytochrome c Oxidase Assembly, Is a Cu(I)-binding Protein J. Biol. Chem., August 16, 2002; 277(34): 31237 - 31242. [Abstract] [Full Text] [PDF] |
||||
![]() |
T.-S. Tsao, H. E. Murrey, C. Hug, D. H. Lee, and H. F. Lodish Oligomerization State-dependent Activation of NF-kappa B Signaling Pathway by Adipocyte Complement-related Protein of 30 kDa (Acrp30) J. Biol. Chem., August 9, 2002; 277(33): 29359 - 29362. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. Li, W.-S. Hou, C. R. Escalante-Torres, B. D. Gelb, and D. Bromme Collagenase Activity of Cathepsin K Depends on Complex Formation with Chondroitin Sulfate J. Biol. Chem., August 2, 2002; 277(32): 28669 - 28676. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. W. Sanders, M. Vesanen, N. Schuelke, A. Master, L. Schiffner, R. Kalyanaraman, M. Paluch, B. Berkhout, P. J. Maddon, W. C. Olson, et al. Stabilization of the Soluble, Cleaved, Trimeric Form of the Envelope Glycoprotein Complex of Human Immunodeficiency Virus Type 1 J. Virol., July 29, 2002; 76(17): 8875 - 8889. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Schulke, M. S. Vesanen, R. W. Sanders, P. Zhu, M. Lu, D. J. Anselma, A. R. Villa, P. W. H. I. Parren, J. M. Binley, K. H. Roux, et al. Oligomeric and Conformational Properties of a Proteolytically Mature, Disulfide-Stabilized Human Immunodeficiency Virus Type 1 gp140 Envelope Glycoprotein J. Virol., June 27, 2002; 76(15): 7760 - 7776. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Yamamoto, L. B. Poole, R. R. Hantgan, and Y. Kamio An Iron-Binding Protein, Dpr, from Streptococcus mutans Prevents Iron-Dependent Hydroxyl Radical Formation In Vitro J. Bacteriol., June 1, 2002; 184(11): 2931 - 2939. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Carulla, C. Woodward, and G. Barany BetaCore, a designed water soluble four-stranded antiparallel {beta}-sheet protein Protein Sci., June 1, 2002; 11(6): 1539 - 1551. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Dyckman and M. G. Fried The Escherichia coli Cyclic AMP Receptor Protein Forms a 2:2 Complex with RNA Polymerase Holoenzyme, in Vitro J. Biol. Chem., May 17, 2002; 277(21): 19064 - 19070. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. E. Turick, L. S. Tisa, and F. Caccavo Jr. Melanin Production and Use as a Soluble Electron Shuttle for Fe(III) Oxide Reduction and as a Terminal Electron Acceptor by Shewanella algae BrY Appl. Envir. Microbiol., May 1, 2002; 68(5): 2436 - 2444. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. N. Patel, S. M. Noble, G. T. Weatherly, A. Tripathy, D. J. Winzor, and G. J. Pielak Effects of molecular crowding by saccharides on {alpha}-chymotrypsin dimerization Protein Sci., May 1, 2002; 11(5): 997 - 1003. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Ceci, L. Giangiacomo, A. Boffi, and E. Chiancone The Mutation K30D Disrupts the Only Salt Bridge at the Subunit Interface of the Homodimeric Hemoglobin from Scapharca inaequivalvis and Changes the Mechanism of Cooperativity J. Biol. Chem., February 22, 2002; 277(9): 6929 - 6933. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Burke and R. Croteau Interaction with the Small Subunit of Geranyl Diphosphate Synthase Modifies the Chain Length Specificity of Geranylgeranyl Diphosphate Synthase to Produce Geranyl Diphosphate J. Biol. Chem., January 25, 2002; 277(5): 3141 - 3149. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. E. Pursglove, T. D. Mulhern, J. P. Mackay, M. G. Hinds, and G. W. Booker The Solution Structure and Intramolecular Associations of the Tec Kinase Src Homology 3 Domain J. Biol. Chem., January 4, 2002; 277(1): 755 - 762. [Abstract] [Full Text] |
||||
![]() |
A. Mukherjee, C. Saez, and J. Lutkenhaus Assembly of an FtsZ Mutant Deficient in GTPase Activity Has Implications for FtsZ Assembly and the Role of the Z Ring in Cell Division J. Bacteriol., December 15, 2001; 183(24): 7190 - 7197. [Abstract] [Full Text] |