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Biophysical Journal 36: 803-809 (1981)
© 1981 the Biophysical Society

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Voltage-dependent conductance induced by alamethicin-phospholipid conjugates in lipid bilayers.

R Latorre, C G Miller and S Quay

ABSTRACT

Alamethicin, a linear 20-amino acid antibiotic, forms voltage-dependent channels in lipid bilayer membranes. We show here that alamethicin-phospholipid conjugates can be prepared by photolysis of unilamellar vesicles containing alamethicin and a phosphatidylcholine analogue with a carbene precursor at the end of the C-2 fatty acyl chain. This result indicates that at least a portion of the alamethicin molecule is in contact with the hydrocarbon moiety of the membrane in the absence of an applied voltage. Furthermore, the alamethicin-phospholipid photoproduct is able to induce a voltage-gated conductance similar to that of natural alamethicin. The importance of these results in terms of mechanisms for channel gating is discussed.






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Copyright © 1981 by the Biophysical Society.