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Biophysical Journal 37: 553-557 (1982)
© 1982 the Biophysical Society

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A Comparison of Resolution-Enhancement Methods in Saturation-Transfer EPR

15N Isotopically Substituted Spin Labels and 35 GHz High-Frequency Operation

Michael E. Johnson, P. Thiyagarajan, Barbara Bates and Bruce L. Currie

ABSTRACT

The use of 15N isotopic substitution and 35 GHz (Q-band) operation as resolution enhancement methods in saturation-transfer electron paramagnetic resonance (ST-EPR) are compared. We find that both methods offer roughly comparable enhancements in ST-EPR resolution. The most powerful approach to resolving complex ST-EPR spectral behavior, however, will probably be the combined use of multiple-frequency X- and Q-band operation with 15N isotopically substituted spin labels.




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E. J. Hustedt and A. H. Beth
High Field/High Frequency Saturation Transfer Electron Paramagnetic Resonance Spectroscopy: Increased Sensitivity to Very Slow Rotational Motions
Biophys. J., June 1, 2004; 86(6): 3940 - 3950.
[Abstract] [Full Text] [PDF]


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Copyright © 1982 by the Biophysical Society.