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Biophysical Journal 38: 315-317 (1982)
© 1982 the Biophysical Society

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The Stability Constant of the 1:1 Complex of Sodium with Guanosine 5'-Monophosphate

Judith A. Walmsley and Thomas J. Pinnavaia

ABSTRACT

The stability of the 1:1 complex of sodium ion with the dianion of guanosine 5'-monophosphate has been determined by means of a potentiometric titration employing a specific ion electrode. The stability constant for the reaction Na+ + 5'-GMP2- Na(5'-GMP)- was found to be 2.85 ± 0.36 M-1 at 5°C and an ionic strength of 1.1 ± 0.1 M. Although 5'-GMP forms ordered self-structures at high concentration in the presence of sodium ions, in dilute solution and at low sodium ion concentrations the Na+ binding is weak and typical of that for other nucleotides.






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Copyright © 1982 by the Biophysical Society.