Biophysical Journal 38: 323-326 (1982)
© 1982 the Biophysical Society

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Direct High-resolution Nuclear Magnetic Resonance Studies of Cation Transport in Vivo

Na⁺ Transport in Yeast Cells

ABSTRACT

A new nuclear magnetic resonance (NMR) method for monitoring transmembrane metal cation transport is reported. It is illustrated with a study of Na⁺ efflux from Na⁺-rich yeast cells. The technique involves the use of an anionic paramagnetic shift reagent, present only outside the cells, to induce a splitting of the sodium-23 NMR peak, in this case, into components representing intra- and extracellular Na⁺. The time course of the efflux is in good agreement with the literature and can be well fitted with a double exponential decay expression. Splitting of the lithium-7 NMR signal from a suspension of Li⁺-rich respiratory-deficient, petite yeasts is also reported.