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Abstract | Full Text | PDF (164 kb) - …more
Copyright © 1964 The Biophysical Society. All rights reserved.
Biophysical Journal, Volume 4, Issue 6, 441-450, 1 November 1964
doi:10.1016/S0006-3495(64)86794-1
Articles
Life Cycle Analysis of Mammalian Cells
T.T. Puck, P. Sanders and D. Petersen
Abstract
A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a total generation time of 12.4 hours as opposed to 20.1 hours for the S3 HeLa cell. However, the relative lengths of each phase of the life cycle are identical within experimental uncertainty in the two cells.
