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Biophysical Journal 44: 107-112 (1983)
© 1983 the Biophysical Society

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Comparison of isotropic calcium signals from intact frog muscle fibers injected with Arsenazo III or Antipyrylazo III.

S M Baylor, M E Quinta-Ferreira and C S Hui

ABSTRACT

Intact single skeletal muscle fibers were micro-injected with either of the metallochromic indicator dyes Arsenazo III or Antipyrylazo III, and dye-related Ca2+ signals from each were measured during a twitch. In comparison with the Arsenazo III Ca2+ signal, the signal from Antipyrylazo III had three favorable features: (a) it was temporally faster, (b) its spectral dependence agreed with a cuvette calibration, and (c) its kinetic behavior was consistent with a single Ca2+ -dye stoichiometry. It is therefore suggested that the Antipyrylazo III Ca2+ signal is a more accurate monitor of the time course of the underlying myoplasmic free Ca2+ transient and one that may be more reliably calibrated.






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Copyright © 1983 by the Biophysical Society.