Biophysical Journal 44: 123-126 (1983)
© 1983 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Connell, G R Articles by Williams, R L Search for Related Content PubMed PubMed Citation Articles by Connell, G R Articles by Williams, R L

Electroimmunoassay. A new competitive protein-binding assay using antibody-sensitive electrodes.

ABSTRACT

A novel technique is reported in which an antibody-sensitive electrode for anti-prostaglandin E2 antisera was used to measure solution-phase PGE2 in nanomolar quantities. The electrode was constructed by incorporating a cation-selective ionophore-hapten (PGE2) conjugate into a polyvinyl chloride membrane. Transmembrane potential in a fixed potassium gradient was measured. The addition of anti-PGE2 antisera changed membrane potential in a concentration-dependent manner. The effect of anti-PGE2 antibodies on membrane potential was decreased by adding free PGE2 to the buffer-containing antisera. With this technique a competitive protein-binding assay was developed, and standard curves for solution-phase PGE2 were generated over a concentration range of 1 to 1,000 nM. The assay was relatively specific for PGE2; PGD2 and PGF2a had only minor effects on transmembrane potential over the effective concentration range for PGE2.