Biophysical Journal 44: 299-304 (1983)
© 1983 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Corson, D W Articles by Fein, A Search for Related Content PubMed PubMed Citation Articles by Corson, D W Articles by Fein, A

Quantitative pressure injection of picoliter volumes into Limulus ventral photoreceptors.

ABSTRACT

Many pharmacological probes must be applied to the interior of cells to produce their effects. Ideally, a method for injecting such materials should be simple, rapid, and independent of the chemical properties of the material to be injected. In addition, one might desire to confirm immediately that an injection occurred and to estimate the volume injected shortly thereafter. We report that these conditions are fulfilled when the injection of materials from micropipettes by pressure pulses is confirmed by visualization of injection-induced disturbances in cells viewed on a video monitor. Volumes of aqueous droplets subsequently injected into a nearby oil pool may be used to estimate the volumes injected into cells. We have obtained a calibration curve for these quantitative estimates of injected volumes by injecting radioactively labeled sulfate into Limulus photoreceptor cells. We find that the estimates are accurate within a range covering one order of magnitude. We assess the sources of systematic and random errors in making these estimates.

This article has been cited by other articles:

				A. Fein Inositol 1,4,5-trisphosphate-induced Calcium Release Is Necessary for Generating the Entire Light Response of Limulus Ventral Photoreceptors J. Gen. Physiol., April 28, 2003; 121(5): 441 - 449. [Abstract] [Full Text] [PDF]

				A. Dabdoub and R. Payne Protein Kinase C Activators Inhibit the Visual Cascade in Limulus Ventral Photoreceptors at an Early Stage J. Neurosci., December 1, 1999; 19(23): 10262 - 10269. [Abstract] [Full Text] [PDF]

				E. A. Richard, S. Ghosh, J. M. Lowenstein, and J. E. Lisman Ca2+/calmodulin-binding peptides block phototransduction in Limulus ventral photoreceptors: Evidence for direct inhibition of phospholipase C PNAS, December 9, 1997; 94(25): 14095 - 14099. [Abstract] [Full Text] [PDF]

				R. Kennedy, M. Oates, B. Cooper, B Nickerson, and J. Jorgenson Microcolumn separations and the analysis of single cells Science, October 6, 1989; 246(4926): 57 - 63. [Abstract] [PDF]

				A Fein Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S Science, June 20, 1986; 232(4757): 1543 - 1545. [Abstract] [PDF]