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Biophysical Journal 46: 195-203 (1984)
© 1984 the Biophysical Society

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Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.

N V Katre, J Finer-Moore, R M Stroud and S B Hayward

ABSTRACT

We located a heavy metal label, mercurilated phenylglyoxal, in both the primary sequence and in the tertiary structure of bacteriorhodopsin. This label modified arginines 225 and 227, which are on the COOH-terminal helix (G). In the projected electron potential difference map, the major site is close to the central inner helix. From this result we conclude that helix 1 could not be the COOH-terminal helix G. We tested the multiple isomorphous replacement method for obtaining phases for purple membrane by electron diffraction.






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Copyright © 1984 by the Biophysical Society.