help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 46: 277-283 (1984)
© 1984 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sine, S M
Right arrow Articles by Steinbach, J H
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sine, S M
Right arrow Articles by Steinbach, J H

Agonists block currents through acetylcholine receptor channels.

S M Sine and J H Steinbach

ABSTRACT

We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.




This article has been cited by other articles:


Home page
 J. Gen. Physiol.Home page
Y. Purohit and C. Grosman
Block of Muscle Nicotinic Receptors by Choline Suggests that the Activation and Desensitization Gates Act as Distinct Molecular Entities
J. Gen. Physiol., May 30, 2006; 127(6): 703 - 717.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
D. Rayes, G. Spitzmaul, S. M. Sine, and C. Bouzat
Single-Channel Kinetic Analysis of Chimeric {alpha}7-5HT3A Receptors
Mol. Pharmacol., November 1, 2005; 68(5): 1475 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Wen and P. Brehm
Paired Motor Neuron-Muscle Recordings in Zebrafish Test the Receptor Blockade Model for Shaping Synaptic Current
J. Neurosci., August 31, 2005; 25(35): 8104 - 8111.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. Akk, L. S Milescu, and M. Heckmann
Activation of heteroliganded mouse muscle nicotinic receptors
J. Physiol., April 15, 2005; 564(2): 359 - 376.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Hallermann, S. Heckmann, J. Dudel, and M. Heckmann
Short openings in high resolution single channel recordings of mouse nicotinic receptors
J. Physiol., March 15, 2005; 563(3): 645 - 662.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. Abdrakhmanova, L. Cleemann, J. Lindstrom, and M. Morad
Differential Modulation of {beta}2 and {beta}4 Subunits of Human Neuronal Nicotinic Acetylcholine Receptors by Acidification
Mol. Pharmacol., August 1, 2004; 66(2): 347 - 355.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Heginbotham and E. Kutluay
Revisiting Voltage-Dependent Relief of Block in Ion Channels: A Mechanism Independent of Punchthrough
Biophys. J., June 1, 2004; 86(6): 3663 - 3670.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
L. K. Lyford, A. D. Sproul, D. Eddins, J. T. McLaughlin, and R. L. Rosenberg
Agonist-Induced Conformational Changes in the Extracellular Domain of {alpha}7 Nicotinic Acetylcholine Receptors
Mol. Pharmacol., September 1, 2003; 64(3): 650 - 658.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
R. J. Prince, R. A. Pennington, and S. M. Sine
Mechanism of Tacrine Block at Adult Human Muscle Nicotinic Acetylcholine Receptors
J. Gen. Physiol., August 26, 2002; 120(3): 369 - 393.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. Rush, A. Kuryatov, M. E. Nelson, and J. Lindstrom
First and Second Transmembrane Segments of alpha 3, alpha 4, beta 2, and beta 4 Nicotinic Acetylcholine Receptor Subunits Influence the Efficacy and Potency of Nicotine
Mol. Pharmacol., June 1, 2002; 61(6): 1416 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. Abdrakhmanova, J. Dorfman, Y. Xiao, and M. Morad
Protons Enhance the Gating Kinetics of the alpha 3/beta 4 Neuronal Nicotinic Acetylcholine Receptor by Increasing Its Apparent Affinity to Agonists
Mol. Pharmacol., February 1, 2002; 61(2): 369 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. D. Philipson, J. P. Gallivan, G. S. Brandt, D. A. Dougherty, and H. A. Lester
Incorporation of caged cysteine and caged tyrosine into a transmembrane segment of the nicotinic ACh receptor
Am J Physiol Cell Physiol, July 1, 2001; 281(1): C195 - C206.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Legendre, D. W. Ali, and P. Drapeau
Recovery from Open Channel Block by Acetylcholine during Neuromuscular Transmission in Zebrafish
J. Neurosci., January 1, 2000; 20(1): 140 - 148.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
P. Legendre
Voltage Dependence of the Glycine Receptor-Channel Kinetics in the Zebrafish Hindbrain
J Neurophysiol, November 1, 1999; 82(5): 2120 - 2129.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Zhang, Y. Xiao, G. Abdrakhmanova, W. Wang, L. Cleemann, K.J. Kellar, and M. Morad
Activation and Ca2+ Permeation of Stably Transfected alpha 3/beta 4 Neuronal Nicotinic Acetylcholine Receptor
Mol. Pharmacol., June 1, 1999; 55(6): 970 - 981.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
J. Dudel, M. Schramm, C. Franke, E. Ratner, and H. Parnas
Block of Quantal End-Plate Currents of Mouse Muscle by Physostigmine and Procaine
J Neurophysiol, May 1, 1999; 81(5): 2386 - 2397.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. A. Forman and Q. Zhou
Novel Modulation of a Nicotinic Receptor Channel Mutant Reveals that the Open State Is Stabilized by Ethanol
Mol. Pharmacol., January 1, 1999; 55(1): 102 - 108.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. A. Stafford, R. E. Oswald, A. Figl, B. N. Cohen, and G. A. Weiland
Two Domains of the Beta Subunit of Neuronal Nicotinic Acetylcholine Receptors Contribute to the Affinity of Substance P
J. Pharmacol. Exp. Ther., August 1, 1998; 286(2): 619 - 626.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. A. Stauderman, L. S. Mahaffy, M. Akong, G. Veliçelebi, L. E. Chavez-Noriega, J. H. Crona, E. C. Johnson, K. J. Elliott, A. Gillespie, R. T. Reid, et al.
Characterization of Human Recombinant Neuronal Nicotinic Acetylcholine Receptor Subunit Combinations alpha 2beta 4, alpha 3beta 4 and alpha 4beta 4 Stably Expressed in HEK293 Cells
J. Pharmacol. Exp. Ther., February 1, 1998; 284(2): 777 - 789.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
W. R. Kem, V. M. Mahnir, R. L. Papke, and C. J. Lingle
Anabaseine Is a Potent Agonist on Muscle and Neuronal Alpha-Bungarotoxin-Sensitive Nicotinic Receptors
J. Pharmacol. Exp. Ther., December 1, 1997; 283(3): 979 - 992.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the Biophysical Society.