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Biophysical Journal 46: 491-495 (1984)
© 1984 the Biophysical Society

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Neutron scattering shows that cytochrome b5 penetrates deeply into the lipid bilayer.

E P Gogol and D M Engelman

ABSTRACT

Cytochrome b5 was asymmetrically reconstituted into small lipid vesicles made of a highly deuterated phospholipid. Small-angle neutron diffraction patterns were collected in a series of H2O-D2O mixtures from vesicles consisting of lipid and native or trypsinized cytochrome b5. The second moment of the radial distribution of scattering density in the vesicles was derived from these data and was compared to values calculated from three proposed models, which differ by the degree that cytochrome b5 penetrates the lipid bilayer. The model in which the hydrophobic domain of the protein is distributed across the bilayer agreed most closely with the data.






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Copyright © 1984 by the Biophysical Society.