Biophysical Journal 46: 831-835 (1984)
© 1984 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Olans, L Articles by Benos, D J Search for Related Content PubMed PubMed Citation Articles by Olans, L Articles by Benos, D J

Saturation behavior of single, amiloride-sensitive Na+ channels in planar lipid bilayers.

ABSTRACT

Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Single-channel conductance was found to be a saturable function of Na+ concentration, with a Michaelis constant of approximately 17 or 47 mM, for a Gmax of approximately 4 or 44 pS, respectively.

This article has been cited by other articles:

				E. Leal-Pinto, A. Teixeira, B. Tran, B. Hanss, and P. E. Klotman Presence of the nucleic acid channel in renal brush-border membranes: allosteric modulation by extracellular calcium Am J Physiol Renal Physiol, July 1, 2005; 289(1): F97 - F106. [Abstract] [Full Text] [PDF]

				V. W. Tang and D. A. Goodenough Paracellular Ion Channel at the Tight Junction Biophys. J., March 1, 2003; 84(3): 1660 - 1673. [Abstract] [Full Text] [PDF]