help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 47: 105-113 (1985)
© 1985 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tamm, L K
Right arrow Articles by McConnell, H M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tamm, L K
Right arrow Articles by McConnell, H M

Supported phospholipid bilayers.

L K Tamm and H M McConnell

ABSTRACT

Phospholipid bilayers have been formed on glass, quartz, and silicon surfaces by a sequential transfer of two monolayers at a pressure of approximately 40 dyn/cm from the air-water interface to the solid substrates. Lateral diffusion measurements of L-alpha-dipalmitoylphosphatidylcholine (DPPC) bilayers supported on oxidized silicon wafers reveal two sharp phase transitions at temperatures similar to those found in multilayer systems with several different techniques. The diffusion measurements obtained using fluorescence recovery after pattern photobleaching provide evidence for the existence of an intermediate (probably P beta' or ripple) phase in single bilayers. While in the intermediate and high temperature (liquid-crystalline L alpha) phase, the diffusion coefficients do not vary very much with temperature, a strong temperature dependence is observed in the low temperature (gel L beta') phase. This is attributed to defect-mediated diffusion. Lipids in silicon supported bilayers made from L-alpha-dioleoylphosphatidylcholine (DOPC) or L-alpha-dimyristoylphosphatidylcholine (DMPC) diffuse rapidly above their respective chain-melting transition temperatures. Arrhenius plots show straight lines with activation energies of 40.9 and 43.7 kJ/mol, respectively. Supported DPPC bilayers on oxidized silicon form long tubular liposomes when heated through their oxidized silicon form long tubular liposomes when heated through their chain-melting-phase transition, as viewed with epifluorescence microscopy. It is suggested that this is a consequence of the expansion of the lipid on the fixed solid support. Conversely, DOPC bilayers form large void areas on this substrate upon cooling. Large circular membrane defects (holes) are observed under rapid coating conditions.(ABSTRACT TRUNCATED AT 250 WORDS)




This article has been cited by other articles:


Home page
 Biophys. JHome page
W. J. Galush, J. A. Nye, and J. T. Groves
Quantitative Fluorescence Microscopy Using Supported Lipid Bilayer Standards
Biophys. J., September 1, 2008; 95(5): 2512 - 2519.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Reich, M. R. Horton, B. Krause, A. P. Gast, J. O. Radler, and B. Nickel
Asymmetric Structural Features in Single Supported Lipid Bilayers Containing Cholesterol and GM1 Resolved with Synchrotron X-Ray Reflectivity
Biophys. J., July 15, 2008; 95(2): 657 - 668.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. C. Anglin and J. C. Conboy
Lateral Pressure Dependence of the Phospholipid Transmembrane Diffusion Rate in Planar-Supported Lipid Bilayers
Biophys. J., July 1, 2008; 95(1): 186 - 193.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. Liu, T. Wang, E. R. Chapman, and J. C. Weisshaar
Productive Hemifusion Intermediates in Fast Vesicle Fusion Driven by Neuronal SNAREs
Biophys. J., February 15, 2008; 94(4): 1303 - 1314.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
X. Chen, J. Wang, C. B. Kristalyn, and Z. Chen
Real-Time Structural Investigation of a Lipid Bilayer during Its Interaction with Melittin Using Sum Frequency Generation Vibrational Spectroscopy
Biophys. J., August 1, 2007; 93(3): 866 - 875.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. R. Cambrea and J. S. Hovis
Formation of Three-Dimensional Structures in Supported Lipid Bilayers
Biophys. J., May 15, 2007; 92(10): 3587 - 3594.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. C. Howland, A. W. Szmodis, B. Sanii, and A. N. Parikh
Characterization of Physical Properties of Supported Phospholipid Membranes Using Imaging Ellipsometry at Optical Wavelengths
Biophys. J., February 15, 2007; 92(4): 1306 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. Kiessling, J. M. Crane, and L. K. Tamm
Transbilayer Effects of Raft-Like Lipid Domains in Asymmetric Planar Bilayers Measured by Single Molecule Tracking
Biophys. J., November 1, 2006; 91(9): 3313 - 3326.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. T. Samiee, J. M. Moran-Mirabal, Y. K. Cheung, and H. G. Craighead
Zero Mode Waveguides for Single-Molecule Spectroscopy on Lipid Membranes
Biophys. J., May 1, 2006; 90(9): 3288 - 3299.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Y. Nishimura, M. Vrljic, L. O. Klein, H. M. McConnell, and W. E. Moerner
Cholesterol Depletion Induces Solid-like Regions in the Plasma Membrane
Biophys. J., February 1, 2006; 90(3): 927 - 938.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. Liu, W. C. Tucker, A. Bhalla, E. R. Chapman, and J. C. Weisshaar
SNARE-Driven, 25-Millisecond Vesicle Fusion In Vitro
Biophys. J., October 1, 2005; 89(4): 2458 - 2472.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. K. Rainey and B. D. Sykes
Optimizing Oriented Planar-Supported Lipid Samples for Solid-State Protein NMR
Biophys. J., October 1, 2005; 89(4): 2792 - 2805.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Liu and J. C. Conboy
1,2-Diacyl-Phosphatidylcholine Flip-Flop Measured Directly by Sum-Frequency Vibrational Spectroscopy
Biophys. J., October 1, 2005; 89(4): 2522 - 2532.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. B. Pedersen, T. Kaasgaard, M. O. Jensen, S. Frokjaer, O. G. Mouritsen, and K. Jorgensen
Phase Behavior and Nanoscale Structure of Phospholipid Membranes Incorporated with Acylated C14-Peptides
Biophys. J., October 1, 2005; 89(4): 2494 - 2503.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Z. V. Leonenko, E. Finot, H. Ma, T. E. S. Dahms, and D. T. Cramb
Investigation of Temperature-Induced Phase Transitions in DOPC and DPPC Phospholipid Bilayers Using Temperature-Controlled Scanning Force Microscopy
Biophys. J., June 1, 2004; 86(6): 3783 - 3793.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. L. Stottrup, S. L. Veatch, and S. L. Keller
Nonequilibrium Behavior in Supported Lipid Membranes Containing Cholesterol
Biophys. J., May 1, 2004; 86(5): 2942 - 2950.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. M. Crane and L. K. Tamm
Role of Cholesterol in the Formation and Nature of Lipid Rafts in Planar and Spherical Model Membranes
Biophys. J., May 1, 2004; 86(5): 2965 - 2979.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M.-C. Giocondi and C. Le Grimellec
Temperature Dependence of the Surface Topography in Dimyristoylphosphatidylcholine/Distearoylphosphatidylcholine Multibilayers
Biophys. J., April 1, 2004; 86(4): 2218 - 2230.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Saccani, S. Castano, B. Desbat, and D. Blaudez
A Phospholipid Bilayer Supported under a Polymerized Langmuir Film
Biophys. J., December 1, 2003; 85(6): 3781 - 3787.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H. X. You, X. Qi, G. A. Grabowski, and L. Yu
Phospholipid Membrane Interactions of Saposin C: In Situ Atomic Force Microscopic Study
Biophys. J., March 1, 2003; 84(3): 2043 - 2057.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. Kiessling and L. K. Tamm
Measuring Distances in Supported Bilayers by Fluorescence Interference-Contrast Microscopy: Polymer Supports and SNARE Proteins
Biophys. J., January 1, 2003; 84(1): 408 - 418.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. E. Saslowsky, J. Lawrence, X. Ren, D. A. Brown, R. M. Henderson, and J. M. Edwardson
Placental Alkaline Phosphatase Is Efficiently Targeted to Rafts in Supported Lipid Bilayers
J. Biol. Chem., July 19, 2002; 277(30): 26966 - 26970.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Desmeules, M. Grandbois, V. A. Bondarenko, A. Yamazaki, and C. Salesse
Measurement of Membrane Binding between Recoverin, a Calcium-Myristoyl Switch Protein, and Lipid Bilayers by AFM-Based Force Spectroscopy
Biophys. J., June 1, 2002; 82(6): 3343 - 3350.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Dietrich, Z. N. Volovyk, M. Levi, N. L. Thompson, and K. Jacobson
Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayers
PNAS, September 4, 2001; (2001) 191168698.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Dietrich, Z. N. Volovyk, M. Levi, N. L. Thompson, and K. Jacobson
From the Cover: Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayers
PNAS, September 11, 2001; 98(19): 10642 - 10647.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Biophysical Society.