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Biophysical Journal 48: 327-329 (1985)
© 1985 the Biophysical Society

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Does Dimeric Melittin Occur in Aqueous Solutions?

D. Schubert, G. Pappert and K. Boss

ABSTRACT

Melittin, a peptide from bee venom, is known to undergo a monomer / tetramer conversion in aqueous solutions. We have studied the possible participation of dimers in the association equilibrium of melittin by sedimentation equilibrium experiments in the analytical ultracentrifuge and subsequent mathematical analysis of the concentration distributions obtained. It was found that the dimeric state is not significantly populated, the contribution of dimer to the total peptide weight probably being below 0.5%.






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Copyright © 1985 by the Biophysical Society.