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Biophysical Journal 5: 231-246 (1965)
© 1965 the Biophysical Society

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Chromatographic Separation of Annealed and Enzymatically Synthesized RNA-DNA Hybrids

Marie N. Hayashi, M. Hayashi and S. Spiegelman

ABSTRACT

A procedure is described for the chromatographic detection and isolation of DNA-RNA hybrids on columns of methylated albumin coated on kieselguhr (MAK). Its use is illustrated with both annealed and enzymatically synthesized hybrids. The method has the advantage of a wide range in capacity and resolution and permits actual isolation of the hybrid structure. It is uniquely effective in experiments involving hybridization with small DNA fragments.






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Copyright © 1965 by the Biophysical Society.