help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 50: 75-80 (1986)
© 1986 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishii, Y
Right arrow Articles by Lehrer, S S
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishii, Y
Right arrow Articles by Lehrer, S S

Effects of the state of the succinimido-ring on the fluorescence and structural properties of pyrene maleimide-labeled alpha alpha-tropomyosin.

Y Ishii and S S Lehrer

ABSTRACT

Rabbit skeletal alpha alpha-tropomyosin was labeled at Cys-190 with pyrene maleimide to form (S-[N-(1-pyrene)succinimido])2-tropomyosin (pyreneI-Tm). The product with cleaved succinimido-rings, pyreneII-Tm was also prepared by incubation of pyreneI-Tm at pH greater than 7.5. The pH dependence of the rate of cleavage indicated that hydrolysis rather than aminolysis was the more likely reaction. PyreneI-Tm exhibited a loss in helix content and end-to-end polymerization compared with unlabeled Tm, which increased upon formation of pyreneII-Tm. The cleavage resulted in increased interchain excited state excimer fluorescence originating from pyrene-pyrene interaction between the chains. Thus, increased pyrene-pyrene interaction at Cys 190 leads to an increase in unfolding, the effects of which appear to be transmitted to the ends of tropomyosin. The fluorescence properties of the two types of pyrene-succinimide adducts of dithiothreitol were very similar to the corresponding adducts of pyrene-Tm indicating excimer formation through ground state pyrene-pyrene interaction.




This article has been cited by other articles:


Home page
 J. Virol.Home page
C. B. Whitehurst, E. J. Soderblom, M. L. West, R. Hernandez, M. B. Goshe, and D. T. Brown
Location and Role of Free Cysteinyl Residues in the Sindbis Virus E1 and E2 Glycoproteins
J. Virol., June 15, 2007; 81(12): 6231 - 6240.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Goransson and D. J. Craik
Disulfide Mapping of the Cyclotide Kalata B1: CHEMICAL PROOF OF THE CYCLIC CYSTINE KNOT MOTIF
J. Biol. Chem., November 28, 2003; 278(48): 48188 - 48196.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
C. R. Borges and J. T. Watson
Recognition of cysteine-containing peptides through prompt fragmentation of the 4-dimethylaminophenylazophenyl-4'-maleimide derivative during analysis by MALDI-MS
Protein Sci., July 1, 2003; 12(7): 1567 - 1572.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Hammarstrom, M. Persson, P.-O. Freskgard, L.-G. Martensson, D. Andersson, B.-H. Jonsson, and U. Carlsson
Structural Mapping of an Aggregation Nucleation Site in a Molten Globule Intermediate
J. Biol. Chem., November 12, 1999; 274(46): 32897 - 32903.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. W. Nadeau, D. B. Sacks, and G. M. Carlson
Differential Affinity Cross-linking of Phosphorylase Kinase Conformers by the Geometric Isomers of Phenylenedimaleimide
J. Biol. Chem., October 17, 1997; 272(42): 26196 - 26201.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the Biophysical Society.