| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 50: 1009-1014 (1986)
© 1986 the Biophysical Society
ABSTRACT
Purified canine cardiac sarcoplasmic reticulum vesicles were passively loaded with 45CaCl2 and assayed for Ca2+ releasing activity according to a rapid quench protocol. Ca2+ release from a subpopulation of vesicles was found to be activated by micromolar Ca2+ and millimolar adenine nucleotides, and inhibited by millimolar Mg2+ and micromolar ruthenium red. 45Ca2+ release in the presence of 10 microM free Ca2+ gave a half-time for efflux of 20 ms. Addition of 5 mM ATP to 10 microM free Ca2+ increased efflux twofold (t1/2 = 10 ms). A high-conductance calcium-conducting channel was incorporated into planar lipid bilayers from the purified cardiac sarcoplasmic reticulum fractions. The channel displayed a unitary conductance of 75 +/- 3 pS in 53 mM trans Ca2+ and was selective for Ca2+ vs. Tris+ by a ratio of 8.74. The channel was dependent on cis Ca2+ for activity and was also stimulated by millimolar ATP. Micromolar ruthenium red and millimolar Mg2+ were inhibitory, and reduced open probability in single-channel recordings. These studies suggest that cardiac sarcoplasmic reticulum contains a high-conductance Ca2+ channel that releases Ca2+ with rates significant to excitation-contraction coupling.
This article has been cited by other articles:
 |
|
 |
|
  J. E. Geiger and N. S. Magoski Ca2+-Induced Ca2+ Release in Aplysia Bag Cell Neurons Requires Interaction Between Mitochondrial and Endoplasmic Reticulum Stores J Neurophysiol, July 1, 2008; 100(1): 24 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Venetucci, A. W. Trafford, S. C. O'Neill, and D. A. Eisner The sarcoplasmic reticulum and arrhythmogenic calcium release Cardiovasc Res, January 15, 2008; 77(2): 285 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Jackson and S. A. Thayer Mitochondrial Modulation of Ca2+-Induced Ca2+-Release in Rat Sensory Neurons J Neurophysiol, September 1, 2006; 96(3): 1093 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yang, S. M. Harrison, and D. S. Steele ATP-dependent effects of halothane on SR Ca2+ regulation in permeabilized atrial myocytes Cardiovasc Res, January 1, 2005; 65(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu and G. Meissner Mechanism of Calmodulin Inhibition of Cardiac Sarcoplasmic Reticulum Ca2+ Release Channel (Ryanodine Receptor) Biophys. J., February 1, 2004; 86(2): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zahradnikova, M. Dura, I. Gyorke, A. L. Escobar, I. Zahradnik, and S. Gyorke Regulation of dynamic behavior of cardiac ryanodine receptor by Mg2+ under simulated physiological conditions Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1059 - C1070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Most, A. Remppis, S. T. Pleger, E. Loffler, P. Ehlermann, J. Bernotat, C. Kleuss, J. Heierhorst, P. Ruiz, H. Witt, et al. Transgenic Overexpression of the Ca2+-binding Protein S100A1 in the Heart Leads to Increased in Vivo Myocardial Contractile Performance J. Biol. Chem., September 5, 2003; 278(36): 33809 - 33817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sanchez, C. Hidalgo, and P. Donoso Kinetic Studies of Calcium-Induced Calcium Release in Cardiac Sarcoplasmic Reticulum Vesicles Biophys. J., April 1, 2003; 84(4): 2319 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Morrissette, L. Xu, A. Nelson, G. Meissner, and B. A. Block Characterization of RyR1-slow, a ryanodine receptor specific to slow-twitch skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1889 - R1898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, A. Tripathy, D. A. Pasek, and G. Meissner Ruthenium Red Modifies the Cardiac and Skeletal Muscle Ca2+ Release Channels (Ryanodine Receptors) by Multiple Mechanisms J. Biol. Chem., November 12, 1999; 274(46): 32680 - 32691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. BERS, L. LI, H. SATOH, and E. McCALL Factors That Control Sarcoplasmic Reticulum Calcium Release in Intact Ventricular Myocytes Ann. N.Y. Acad. Sci., September 16, 1998; 853(1): 157 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kawai, M. Hussain, and C. H. Orchard Cs+ inhibits spontaneous Ca2+ release from sarcoplasmic reticulum of skinned cardiac myocytes Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H422 - H430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.A Eisner, A.W Trafford, M.E Dnaz, C.L Overend, and S.C O'Neill The control of Ca release from the cardiac sarcoplasmic reticulum: regulation versus autoregulation Cardiovasc Res, June 1, 1998; 38(3): 589 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Quinn, L. Castellani, K. Ondrias, and B. E. Ehrlich Characterization of the ryanodine receptor/channel of invertebrate muscle Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R494 - R502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. W. Chen, X. Li, K. Ebisawa, and L. Zhang Functional Characterization of the Recombinant Type 3 Ca2+ Release Channel (Ryanodine Receptor) Expressed in HEK293 Cells J. Biol. Chem., September 26, 1997; 272(39): 24234 - 24246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zucchi and S. Ronca-Testoni The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States Pharmacol. Rev., March 1, 1997; 49(1): 1 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kaftan, A. R. Marks, and B. E. Ehrlich Effects of Rapamycin on Ryanodine Receptor/Ca2+-Release Channels From Cardiac Muscle Circ. Res., June 1, 1996; 78(6): 990 - 997. [Abstract] [Full Text]
|
|
|
|
|
|
R. Sitsapesan, R. A.P. Montgomery, and A. J. Williams New Insights Into the Gating Mechanisms of Cardiac Ryanodine Receptors Revealed by Rapid Changes in Ligand Concentration Circ. Res., October 1, 1995; 77(4): 765 - 772. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Valdivia, J. Kaplan, G. Ellis-Davies, and W. Lederer Rapid adaptation of cardiac ryanodine receptors: modulation by Mg2+ and phosphorylation Science, March 31, 1995; 267(5206): 1997 - 2000. [Abstract] [PDF]
|
|
|
|
|
|
E. V. Menshikova, E. Cheong, and G. Salama Low N-Ethylmaleimide Concentrations Activate Ryanodine Receptors by a Reversible Interaction, Not an Alkylation of Critical Thiols J. Biol. Chem., November 17, 2000; 275(47): 36775 - 36780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ohki, T. Miyoshi, M. Murata, K. Ishibashi, M. Imai, and M. Suzuki A Calcium-activated Cation Current by an Alternatively Spliced Form of Trp3 in the Heart J. Biol. Chem., December 8, 2000; 275(50): 39055 - 39060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yang and D. S. Steele Effects of phosphocreatine on SR Ca2+ regulation in isolated saponin-permeabilized rat cardiac myocytes J. Physiol., March 15, 2002; 539(3): 767 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yang and D. S. Steele Effects of Cytosolic ATP on Ca2+ Sparks and SR Ca2+ Content in Permeabilized Cardiac Myocytes Circ. Res., September 14, 2001; 89(6): 526 - 533. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
