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Biophysical Journal 50: 993-997 (1986)
© 1986 the Biophysical Society

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Time-Resolved Structural Studies on Insect Flight Muscle after Photolysis of Caged-ATP

G. Rapp, K. J. V. Poole, Y. Maeda, K. Güth, J. Hendrix and R. S. Goody

ABSTRACT

The time course of structural changes occurring on ATP-induced relaxation of glycerinated insect flight muscle from the rigor state has been investigated using synchrotron radiation as a source of high intensity x rays and photolysis of caged-ATP to produce a rapid rise in ATP concentration. Temporal resolutions of 1 ms for the strongest equatorial reflections and 5 ms for the 14.5 nm meridional reflection are attainable from single events (i.e., without averaging over several cycles). The equatorial intensity changes completely, the meridional intensity partially, towards their respective relaxed values on a much faster time scale than relaxation of tension. The results suggest that actively cycling bridges present shortly after ATP-release are either too few in number to be detected in the equatorial diffraction pattern or that their structure is different from that of rigor bridges.






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Copyright © 1986 by the Biophysical Society.