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Biophysical Journal 51: 383-393 (1987)
© 1987 the Biophysical Society

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In vitro calibration of the equilibrium reactions of the metallochromic indicator dye antipyrylazo III with calcium.

S Hollingworth, R W Aldrich and S M Baylor

ABSTRACT

The equilibrium reactions of the metallochromic indicator dye Antipyrylazo III with calcium at physiological ionic strength have been investigated spectrophotometrically. Dye absorbance as a function of wavelength was measured at various total dye and calcium concentrations. Analysis of the absorbance spectra indicated that at pH 6.9 at least three calcium:dye complexes form, with 1:1, 1:2, and possibly 2:2 stoichiometries. The dissociation constant and the changes in dye extinction coefficients on formation of the 1:2 complex, the main complex which forms when Antipyrylazo III is used to study cytoplasmic calcium transients, have been characterized.






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Copyright © 1987 by the Biophysical Society.