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Biophysical Journal 51: 993-997 (1987)
© 1987 the Biophysical Society

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Quasi-elastic light scattering studies of membrane motion in single red blood cells.

R B Tishler and F D Carlson

ABSTRACT

Studies of red blood cells (RBCs) and RBC ghosts, using a quasi-elastic light scattering (QELS) microscope spectrometer, have identified the membrane as the primary source of the light scattering signal. This is the first report in which motion of the cell membrane has been demonstrated to be the primary source of the QELS signal from a cell. Cytoplasmic changes induced in the RBC by varying the osmotic strength of the medium were also detected using this technique. Comparison of the data from white blood cells (WBCs) with the RBC data demonstrated significant differences between different types of cells.






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Copyright © 1987 by the Biophysical Society.